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Anti Autotaxin/ENPP2 Polyclonal Antibody

Background

Autotaxin, a member of the ENPP (Ectonucleotide pyrophospatase/phosphodiesterase) family of ectoenzymes, was originally identified as a motogen secreted by a melanoma cell line and subsequently reported to promote cell proliferation, cell motility, angiogenesis, and neurite retraction. Autotaxin has lysophospholipase D activity and appears to exert its effects by generation of the lipid mediator lysophosphatidic acid (LPA) from lysophosphatidylcholine and sphingosine 1-phosphate (S1P) from sphingosylphosphorylcholine (SPC) outside the cell.
Autotaxin levels are significantly higher in cancerous tissues than in normal tissues suggesting that this novel extracellular protein may be involved in tumor progression.

Anti Autotaxin/ENPP2 Polyclonal Antibody

[Specifically reacts with a C-terminal fragment of Autotaxin]

Pacage Size 25g (250L/vial)
Format Rabbit polyclonal antibody (0.1mg/mL)
Buffer PBS [containing 50% Block Ace as a stabilizer, 0.1%Proclin as a bacteriostat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of a rabbit immunized with a peptide representing the C-terminal domain of Autotaxin.
Purified by peptide affinity chromatography.
Working dilution For Immunohistochemistry:0.2~2.0g/ml


Immunohistochemistry
Sample:
A) Mouse Spinal cord (Embryonic day 9.5)
B) Mouse Thalamus (Adult)

Preparation of antibodies and instruction:
Masu M.
Koike S.
University of Tsukuba
Graduate School of Comprehensive Human Sciences

Anti Autotaxin/ENPP2 Polyclonal Antibody

[Specifically reacts with an N-terminal fragment of Autotaxin]

Pacage Size 25g (250L/vial)
Format Rabbit polyclonal antibody (0.1mg/mL)
Buffer PBS [containing 50% Block Ace as a stabilizer, 0.1%Proclin as a bacteriostat]
Storage Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of a rabbit immunized with a peptide representing the C-terminal domain of Autotaxin.
Purified by peptide affinity chromatography.
Working dilution For Western blotting:0.5g/ml


Western blotting
Sample: Conditioned medium from COS7 cells
(1) Autotaxin-trasfected cells
(2) Negative control cells

Preparation of antibodies and instruction:
Masu M.
Koike S.
University of Tsukuba
Graduate School of Comprehensive Human Sciences

Product List

Product Name Cat# Quantity Price

Anti Autotaxin / ENPP2

KAL-KM105 25UG

¥ 49,000
$ 654
€ 490

Anti Autotaxin / ENPP2

KAL-KM106 25UG

¥ 49,000
$ 654
€ 490