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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti ASH2L [ASH2, ASH2L1, ASH2L2] monoclonal antibody (clone : 2046D2a)

Background

Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. May function as a transcriptional regulator. May play a role in hematopoiesis. [UniProtKB Function]

Application

[Western Blot]
Detection of ASH2L by Western blot. Samples: Whole cell lysate from human HeLa (H, 50 μg) , mouse NIH3T3 (M, 50 μg) and rat F2408 (R, 50 μg) cells. [Lot No. 2046D2a-1]

Predicted molecular weight: 68 kDa.

Western_blot_protocolPDF

 

[Immunoprecipitation]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ASH2L mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2046D2a-1]

Immunoprecipitation protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ASH2L mAb. [Lot No. 2046D2a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti ASH2L

CBX-CBX00359 100UG

¥ 39,000
$ 520
€ 390