Fig.1. Expression of ACE3 in Testis and sperm of mouse as identified by western blotting with anti-ACE3 antibody. All samples were loaded at 30 µ‚‡ protein and detected with anti-ACE3 antibody at 1/500 dilution.
Fig.2. Immunoprecipitation of ACE3 protein from sperm lysate. ACE3 protein was immunoprecipitated from total sperm protein (~100 µg) with anti-ACE3 antibody and detected by western blotting with anti-ACE3 antibody.
1. Non-immune serum.
2. Anti-ACE3 antibody
Fig.3. Subcellular localization of ACE3 protein in sperm. Mouse sperm were prepared from cauda epidymis, fixed with ethanol and stained with anti-ACE3 antibody at 1/100. As a secondary antibody, Alexa Fluor 546 conjugated anti-rabbit IgG.