Tau is a microtubule-associated protein. In adult human brain, 6 tau isoforms are expressed, which are divided into two groups, 3-repeat (3R) and 4-repeat (4R) tau isoforms. Tau assembles into insoluble, filamentous, and hyperphosphorylated inclusions in many neurodegenerative diseases, and different tau isoforms are accumulated in the diseased brains, namely, 6 tau isoforms in AD, 3R tau isoforms in Pick’s disease, and 4R tau isoforms in PSP and CBD. Therfore, isoform-specific tau antibodies are useful tools for immunohistochemical and biochemical studies of tau species in diseased brains. Tau deposited in AD is extensively deamidated at N279. This anti-4R specifically recognizes 4R tau isoforms regardless of deamidation and strongly stained tau in AD brain.
・Western blotting: 1/2000 - 1/5000
・Immunohistochemistry: 1:3000 - 1/20000
Fig1: Immuno blot analysis for 6 kinds of tau isoforms (3-repeat and 4-repeat) with anti total tau (T46) and Anti 4R (TIP-4RT-P01).
Fig2: Immuno staining for paraffin embedded section of cerebral cortex prepared from AD brain (retrieved with proteinase K and formic acid)
(Fig2 Staining protocol)
After autoclaving (Ac) and formic acid (FA) treatment (70~100 % formic acid for 10 min), sections were treated with 10 micro g/mL Proteinase K (Pro-K, diluted in 30mM Tris buffer pH7.5) for 30 min. After blocking with 10% calf serum/PBS, sections were incubated with the anti-4R antibody (1:1000 diluted in 10% calf serum/PBS) at room temperature over night. After washing with PBS, sections were incubated with biotinylated ant-rabbit 2nd antibody at RT for 1h, incubated with ABC complex for 30 min and developed with DAB /H2O2.
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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.