PRODUCT CATEGORY

PRODUCT SEARCH

NEWSLETTER

Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

Anti 20S proteasome (GC4/5) antibody

Background

The 26S proteasome is an essential component of the ubiquitin-proteolytic pathway in eukaryotic cells and is responsible for the degradation of most cellular proteins. It is composed of a 20S proteasome as a catalytic core and regulatory particles at either end. The subunits of the 20S proteasome can be classified into two families, α and β. In eukaryotes, the 20S proteasome contains seven α-type subunits and seven β-type subunits. The fourteen subunits are arranged in four rings of seven and form an α7β7β7α7 structure. This antibody recognizes α2 subunit of the 20S proteasome from all organisms tested, yeast to human. The advance of this antibody is application for immuno-electron microscopy.

Applications

Figure 1. Immunoblotting of the purified 26S proteasomes

26S proteasomes were electrophoresed under denaturing conditions (12.0% gel) and stained with Coomassie Brilliant Blue (CBBR), or immunostained with antibodies (α-20S, anti-Xenopus 20S proteasome polyclonal antibody; GC3α; GC4/5; GC3β; or 1-4D5) after electroblotting. Lanes I and M indicate 26S proteasomes from immature and mature oocytes, respectively. Protein bands that cross-reacted with GC4/5 (p25), GC3β (p30 and p31) and 1-4D5 (p62) are indicated. Molecular masses of standard proteins are indicated on the left. Reference : Eur J Biochem. 2000 Jan;267(1)97-103

 

Anti 20S proteasome antibody

 

Figure 2. Immunoelectronmicroscopic localization of proteasome α2 subunit

    back to "Anti 20S Proteasome Antibodies" page

Product List

Product Name Cat# Quantity Price

Anti 20S proteasome (GC4/5)

CAC-SZU-PS-M03 100UL

¥ 50,000
$ 667
€ 500

Citation
  • Cytoplasmic lipid droplets are sites of convergence of proteasomal and autophagic degradation of apolipoprotein B.
    Ohsaki Y, Cheng J, Fujita A, Tokumoto T, Fujimoto T.
    Mol Biol Cell 17 2674-2683 (2006) PMID: 16597703


References
  • Identification of the Xenopus 20S proteasome alpha4 subunit which is modified in the meiotic cell cycle. Tokumoto M. et. al., Gene 239 301-308 (1999) PMID: 10548731
  • Two proteins, a goldfish 20S proteasome subunit and the protein interacting with 26S proteasome, change in the meiotic cell cycle. Tokumoto M. et. al., Eur J Biochem 267 97-103 (2000) PMID: 10601855
  • Identification of alpha-type subunits of the Xenopus 20S proteasome and analysis of their changes during the meiotic cell cycle. Wakata Y. et. al., BMC Biochem 5 18 (2004) PMID: 15603592
  • Possible function of caudal nuclear pocket: degradation of nucleoproteins by ubiquitin-proteasome system in rat spermatids and human sperm. Haraguchi CM. et. al., J Histochem Cytochem 55 585-595 (2007) PMID: 17312012

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.