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For research on sperm fertility

Anti-Calreticulin-3 / CALR3 / Calsperin

Background

Calnexin (CANX) and calreticulin (CALR) are homologous lectin chaperones located in the endoplasmic reticulum and cooperate to mediate nascent glycoprotein folding1. Calreticulins are proteins which bind Ca2+ ions which are second messengers in signal transduction.

The protein, Calsperin is coded by the gene CALR3, is a testis-specific chaperone required for sperm fertility. 

CALR3 capacity for calcium-binding may be absent or much lower than that of CALR. During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility.

 

1Ikawa M., et al. Calsperin is a testis-specific chaperone required for sperm fertility. J Biol Chem. 2011 Feb 18;286(7):5639-46. PMID: 21131354

Applications

 

Fig.1. Testis specific expression of Calreticulon-3 as examined in various tissues by western blotting with anti-CALR3 antibody. The various tissues were excised and homogenized in lysis buffer containing 1% TritonX100 and then placed on ice for 1 h. These extracts were centrifuged, and the supernatants were collected and analyzed by western blotting with anti-CALR3 antibody at 1/1,000 dilution.

1. Brain.
2. Lung.
3. Heart.
4. Thymus.
5. Liver.
6. Spleen.
7. Kidney.
8. Muscle.
9. Ovary.
10. Testis.
11. Sperm

 

Fig.2. Identification of Calreticulon-3 protein by western blotting with anti-CALR3 antibody. Embryonic fibroblast cells prepared from Calr3 -/- mouse were transfected with a plasmid expressing Calr3. The cell lysate was analyzed by western blotting with anti-CALR3 antibody at 1/1,000 dilution.
1. Mock-infected cell lysate as a negative control.
2. Cell lysate transfected with a plasmid expressing Calr3.

 

Fig.3 Immunoprecipitation of Calreticulon-3 protein with anti-CALR3 antibody. Lysates of wild-type mouse testis were immunoprecipitated with anti-CALR3 antibody and the precipitates were analyzed by western blotting with the same antibody.

1. Input testis lysate
2. Precipitated with preimmune serum
3. Precipitated with anti-CALR3 antibody

Fig.4 Immunofluorescence staining of a testicular section with anti-CALR3 antibody.Sequential sections were stained with hematoxylin and eosin (upper panel). CALR3 was detected in elongating spermatids (lower panel). Testis was collected from adult mouse and fixed in 4% paraformaldehyde/PBS overnight at 4 ?‹C, cryopreserved in graded 10?30% sucrose, and embedded. Frozen sections (8 µm) were mounted on aminopropyl triethoxysilane-coated glass slides. Primary antibody was used at 1/100 and as secondary antibody, Alexa Fluor 488 conjugated goat anti-rabbit IgG was used. Scale bar is 200 µm.

Product List

Product Name Cat# Quantity Price

Anti Calreticulin-3/CALR3/Calsperin

BAM-73-022-EX 100UL

¥ 34,500
$ 460
€ 345

References
  •  Ikawa M. et al ; Calsperin is a testis-specific chaperone required for sperm fertility. J Biol Chem. 2011 Feb 18;286(7):5639-46. doi: 10.1074/jbc.M110.140152. Epub 2010 Dec 3.: PMID: 21131354 (WB)

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.