SGE-CFT45325 is prepared to stain human cryostat sections of renal and skin biopsy specimens by direct immunofluorescence.
How to stain human kidney cryostat sections
Just put 20-50 µl of the staining solution on cryostat sections, incubate them for 30 min at room temperature. After washing them with PBS, mount them and observe them with a fluorescence microscope.
How to stain human skin cryostat sections
After acetone treatment of cryostat sections for 5 min, put 20-50 µl of the staining solution on them, and incubate them for 60 min in a moisture chamber at room temperature. After washing them with PBS, mount them and observe them with a fluorescence microscope.
In case that a section is not stained or stained in a mosaic pattern with FITC-anti-α5(IV) but the background staining of Texas Red-anti-α2(IV) is normally stained. The disease is diagnosed as Alport's syndrome.
Fluorochrome-conjugated Anti Collagen IV cocktail for Alport's syndrome
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.