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HEL may be a useful biomarker for initial stage of lipid peroxidation

Hexanoyl-Lys (HEL) ELISA Kit and related product


Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydessuch as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HELis formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.
HEL is formed by the reaction of linoleic acid hydroperoxide and Lysine, and a biomarker for oxidative stress. HEL ELISA kit is a competitive enzyme-linked immunosorbent assay for quantitative measurement of hexanoyl-Lys adduct. This kit is based on the monoclonal antibody clone 5H4, which is specific for HEL. Suitable for urine, serum and other biological samples.


Assay Procedure

Kit component

1. HEL Microtiter Plate Precoated with HEL (8x12 wells, Split Type) 1 Plate
2. Primary Antibody Monoclonal Antibody specific for HEL 1 Vial (7ml)
3. Secondary Antibody HRP-Conjugated Anti Mouse IgG Antibody 1 Vial
4. Secondary Antibody Buffer Phosphate Buffered Saline 1 Vial (12ml)
5. Chromogen 3,3',5,5'-Tetramethylbenzidine 1 Vial (250 µL)
6. Chromogen Buffer Hydrogen Peroxide/ Citrate-Phosphate Buffer 1 Vial (12ml)
7. Washing Buffer (5X) Concentrated Phosphate Buffered Saline 2 Vials (25mL X 2)
8. Stop Solution 1M Phosphoric acid 1 Vial (12mL)
9. *Standard A-F Bz-Gly-Hexanoyl-Lys 1 Vial each(500µL)
10. Plate Seal Adhesive seal to prevent evaporation 2 sheets

*(A: 2.6, B:7.7, C:22.7, D:69.7, E:207, F:624 nmol/L)

Standard curve example

Product List

Product Name Cat# Quantity Price

Hexanoyl-Lys(HEL) ELISA kit


¥ 76,800
$ 1024
€ 768

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