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Clone SRA-E5

Anti MSR-A / CD204 Monoclonal Antibody

Backgroud

CD204, also referred as class A macrophage scavenger receptor class A type I/II (MSR-A) or macrophage scavenger receptor type I/II (MSR1), is one of the major receptors of macrophages and plays important roles in many macrophage-related disorders. CD204 binds various negatively charged macromolecules, such as modified low-density lipoproteins, fucoidan, advanced glycation end-products, denatured collagen, and bacterial components.

Application notes

Recommended dilutions
  • Western blot: 3-10 µg/ml (reduced condition)
  • Immunohistochemistry (IHC, IHC-P): 1-10 µg/ml
  • Flow cytometry: 1-5 µg/ml (Permeabilization is recommended)

*Other applications have not been tested.
*Optimal dilutions/concentrations should be determined by the end user.

Antibody Characterization


Figure 1: Immunohistochemistry of CD204 in human colon cancer and lymphoma (Paraffin sections).
There are so many CD204-positive macrophages in stroma of colon cancer (left) and lymphoma (right).
Antigen retrieval: 1mM EDTA buffer (pH8.0), heat (microwave for 5 min)


Figure 2: Western blot analysis of CD204 in human monocyte-derived macrophages.
Predicted molecular weight of CD204 is 43 kDa, however, molecular mass is approximately 50-70 kDa due to glycosylation under reducing condition.
Pre-treatment with N-glycosidase is recommended.
Lane 1: Control human monocyte-derived macrophages
Lane 2: Macrophages stimulated with IL-4
Lane 3: Macrophages stimulated with IL-10
Lane 4-6: Macrophages stimulated with conditional medium of breast cancer cell lines

Product List

Product Name Cat# Quantity Price

Anti MSR-A / CD204

CAC-KMU-MA01 50UG

¥ 50,000
$ 667
€ 500

Citation
  • CD163-positive cancer cells are potentially associated with high malignant potential in clear cell renal cell carcinoma.
    Ma C, Horlad H, Ohnishi K1, Nakagawa T, Yamada S, Kitada S, Motoshima T, Kamba T, Nakayama T, Fujimoto N, Takeya M, Komohara Y.
    Med Mol Morphol. 2017 Jul 7. PMID: 28687956

References
  • Contribution of Macrophage Polarization to Metabolic Diseases.
    Komohara Y, Fujiwara Y, Ohnishi K, Shiraishi D, Takeya M.
    J Atheroscler Thromb. 2016;23(1):10-7. PMID: 26412584
  • Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis.
    Higashi-Kuwata N, Jinnin M, Makino T, Fukushima S, Inoue Y, Muchemwa FC, Yonemura Y, Komohara Y, Takeya M, Mitsuya H, Ihn H.
    Arthritis Res Ther. 2010;12(4):R128. PMID: 20602758
  • Production, characterization, and interspecies reactivities of monoclonal antibodies against human class A macrophage scavenger receptors.
    Tomokiyo Ri, Jinnouchi K, Honda M, Wada Y, Hanada N, Hiraoka T, Suzuki H, Kodama T, Takahashi K, Takeya M.
    Atherosclerosis. 2002 Mar;161(1):123-32. PMID: 11882324
  • A role for macrophage scavenger receptors in atherosclerosis and susceptibility to infection.
    Suzuki H, Kurihara Y, Takeya M, Kamada N, Kataoka M, Jishage K, Ueda O, Sakaguchi H, Higashi T, Suzuki T, Takashima Y, Kawabe Y, Cynshi O, Wada Y, Honda M, Kurihara H, Aburatani H, Doi T, Matsumoto A, Azuma S, Noda T, Toyoda Y, Itakura H, Yazaki Y, Kodama T, et al.
    Nature. 1997 Mar 20;386(6622):292-6. PMID: 9069289