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Clone SRA-E5

Anti MSR-A / CD204 Monoclonal Antibody


CD204, also referred as class A macrophage scavenger receptor class A type I/II (MSR-A) or macrophage scavenger receptor type I/II (MSR1), is one of the major receptors of macrophages and plays important roles in many macrophage-related disorders. CD204 binds various negatively charged macromolecules, such as modified low-density lipoproteins, fucoidan, advanced glycation end-products, denatured collagen, and bacterial components.

Application notes

Recommended dilutions
  • Western blot: 3-10 µg/ml (reduced condition)
  • Immunohistochemistry (IHC, IHC-P): 1-10 µg/ml
  • Flow cytometry: 1-5 µg/ml (Permeabilization is recommended)

*Other applications have not been tested.
*Optimal dilutions/concentrations should be determined by the end user.

Antibody Characterization

Figure 1: Immunohistochemistry of CD204 in human colon cancer and lymphoma (Paraffin sections).
There are so many CD204-positive macrophages in stroma of colon cancer (left) and lymphoma (right).
Antigen retrieval: 1mM EDTA buffer (pH8.0), heat (microwave for 5 min)

Figure 2: Western blot analysis of CD204 in human monocyte-derived macrophages.
Predicted molecular weight of CD204 is 43 kDa, however, molecular mass is approximately 50-70 kDa due to glycosylation under reducing condition.
Pre-treatment with N-glycosidase is recommended.
Lane 1: Control human monocyte-derived macrophages
Lane 2: Macrophages stimulated with IL-4
Lane 3: Macrophages stimulated with IL-10
Lane 4-6: Macrophages stimulated with conditional medium of breast cancer cell lines

Product List

Product Name Cat# Quantity Price

Anti MSR-A / CD204


¥ 50,000
$ 667
€ 500

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