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Clone SRA-E5

Anti MSR-A / CD204 Monoclonal Antibody

Backgroud

CD204, also referred as class A macrophage scavenger receptor class A type I/II (MSR-A) or macrophage scavenger receptor type I/II (MSR1), is one of the major receptors of macrophages and plays important roles in many macrophage-related disorders. CD204 binds various negatively charged macromolecules, such as modified low-density lipoproteins, fucoidan, advanced glycation end-products, denatured collagen, and bacterial components.

Application notes

Recommended dilutions
  • Western blot: 3-10 µg/ml (reduced condition)
  • Immunohistochemistry (IHC, IHC-P): 1-10 µg/ml
  • Flow cytometry: 1-5 µg/ml (Permeabilization is recommended)

*Other applications have not been tested.
*Optimal dilutions/concentrations should be determined by the end user.

Antibody Characterization


Figure 1: Immunohistochemistry of CD204 in human colon cancer and lymphoma (Paraffin sections).
There are so many CD204-positive macrophages in stroma of colon cancer (left) and lymphoma (right).
Antigen retrieval: 1mM EDTA buffer (pH8.0), heat (microwave for 5 min)


Figure 2: Western blot analysis of CD204 in human monocyte-derived macrophages.
Predicted molecular weight of CD204 is 43 kDa, however, molecular mass is approximately 50-70 kDa due to glycosylation under reducing condition.
Pre-treatment with N-glycosidase is recommended.
Lane 1: Control human monocyte-derived macrophages
Lane 2: Macrophages stimulated with IL-4
Lane 3: Macrophages stimulated with IL-10
Lane 4-6: Macrophages stimulated with conditional medium of breast cancer cell lines

Product List

Product Name Cat# Quantity Price

Anti MSR-A / CD204

CAC-KMU-MA01 50UG

¥ 50,000
$ 667
€ 500

References
  • Suzuki H.,et al., : A role for macrophage scavenger receptors in atherosclerosis and susceptibility to infection.Nature. 1997 Mar 20;386(6622):292-6. PMID: 9069289
  • Tomokiyo Ri,et al., : Production, characterization, and interspecies reactivities of monoclonal antibodies against human class A macrophage scavenger receptors. Atherosclerosis. 2002 Mar;161(1):123-32.PMID: 11882324
  • Komohara Y.,et al., : Contribution of Macrophage Polarization to Metabolic Diseases. J Atheroscler Thromb. 2016;23(1):10-7. doi: 10.5551/jat.32359. Epub 2015 Sep 28 PMID: 26412584
  • Higashi-Kuwata N.,et al., : Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis.Arthritis Res Ther. 2010;12(4):R128. doi: 10.1186/ar3066. Epub 2010 Jul 5.PMID: 20602758