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This assay recognizes recombinant and natural rat Kim-1.

(ALPHA)-Synuclein Aggregation Assay Kit

α-Synuclein Aggregation Assay Kit mimics intracellular aggregation of "α-Synuclein" seen in Lewy Body Dementia / Parkinson Disease / Multiple system atrophy.
Cell aggregation inhibition and/or preventive effects of your cell culture assay can be tested with this kit.

Go to "Antibodies for Neurodegenerative Disease Research"

Background

α-Synuclein Aggregation Assay KitDementia is a disease that causes memory and judgement loss, there is no fundamental treatment developed for it. Years of scientific research has proven that protein aggregation occurs in the degeneration part of the brain for many neurodegenerative diseases including dementia, indicating appearance of this aggregation is closely related to these diseases development and progression.

Aggregation occurs when normal protein forms abnormal structure for particular reasons and accumulates in the cell, which each disease has different aggregating protein. It is known that protein called "α-Synuclein" forms abnormal structure in Parkinson Disease / Lewy Body Dementia / Multiple system atrophy patient brain.

α-Synuclein Aggregation Assay Kit mimics intracellular aggregation of "α-Synuclein", which allows in vitro screening of active ingredients.

Kit component

ComponentsVolumeQuantityStorage
pCMV-SNCA (α - Synuclein Expression plasmid vector, Red cap) 
(conc. 1.25 μg/μL)
32 μL 1 vial 4°C
do not freeze
pCMV-NC(Negative control vector, Green cap)
( conc. 1.25 μg/μL)
5 μL 1 vial
pCMV-dGFP(dGFP Expression plasmid vector, Blue cap)
(conc. 1.25 μg/μL)
5 μL 1 vial
20 mM Tris-HCl Buffer (pH7.4) 10 mL 1 bottle
F-αSyn (α - Synuclein Fibrils, Yellow cap)
(conc. 1 μg/μL)
32 μL 1 vial
Multifectam (Gene transfection reagent) 0.33 mg 1 bottle

Overview of the assay flow

overview


overview

Detection example of α -Synuclein aggregation complex

1. Western blotting (Use of various synuclein antibodies)

  1. Remove culture medium.
  2. Add 1 mL PBS to each well, collect cells by pipetting.
  3. Centrifuge (4,500 rpm [1,800 x g], 5 min., room temp.), collect pellet.
  4. Add 0.1 mL Lysis Buff er (10 mM Tris-HCl, pH 7.5 containing 0.8 M NaCl, 1 mM ethyleneglycol bis(2-aminoethyl ether)-N,N,N,N-tetraacetic acid (EGTA), 1 mM DTT and 1% N-Lauroylsarcosine sodium salt ), sonicate mixture.
  5. Centrifuge (50,000 rpm [100,000 x g], 20 min., room temp.).
  6. Add 40 µL 2 x SDS Buffer (0.125 M Tris-HCl pH6.8, 200 mM 1,4 dithiothreitol (DTT), 4% sodium dodecyl sulfate(SDS) , 10% Sucrose, 0.01% BPB) to pellet, sonicate mixture, heat (100 °C,5 min), apply 10 µL/well for western blotting.

overview



2. Amyloid structure fluorescent staining kit

By use of Amyloid Structure Fluorescent Staining Kit (Cosmo Bio, cat. no. CSR-SYN02-COS), dual staining of aggregated α-Synuclein and nucleolus can be performed.
overview

Product List

Product Name Cat# Quantity Price

(ALPHA)-Synuclein Aggregation Assay Kit

CSR-SYN01-COS 1KIT

¥ 110,400
$ 1472
€ 1104

Amyloid Fluorescent Staining Kit

CSR-SYN02-COS 1KIT

¥ 72,000
$ 960
€ 720

(ALPHA)-Synuclein Fibrils

CSR-SYN03-COS 0.1MG

¥ 120,000
$ 1600
€ 1200

(ALPHA)-Synuclein

CSR-SYN04-COS 1MG

¥ 108,000
$ 1440
€ 1080

(ALPHA)-Synuclein

CSR-SYN04-COS 0.1MG

¥ 36,000
$ 480
€ 360

References
  • Nonaka T. et al. J Biol Chem. 2010 Nov 5;285(45):34885-98.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.