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Anti 20S Proteasome Antibodies

Background

The 26S proteasome is an essential component of the ubiquitin-proteolytic pathway in eukaryotic cells and is responsible for the degradation of most cellular proteins. It is composed of a 20S proteasome as a catalytic core and regulatory particles at either end. The subunits of the 20S proteasome can be classified into two families, α and β. In eukaryotes, the 20S proteasome contains seven α-type subunits and seven β-type subunits. The fourteen subunits are arranged in four rings of seven and form an α7β7β7α7 structure. This antibody recognizes α2 subunit of the 20S proteasome from all organisms tested, yeast to human. The advance of this antibody is application for immuno-electron microscopy

Ubiquitylation

Applications

20S proteasome antibody
Figure 1. Localization analysis of 26S proteasome subunits using clone: GC3α; GC4/5; GC3β; or 1-4D5..

20S proteasome antibody
Figure 2. Immunofluorescence staining of rat epididymal sperm and human ejaculated sperm.
(A) Smear preparation of rat sperm. (B) Human ejaculated sperm. Bar = 0.5 µm.

20S proteasome antibody
Figure 3. Immunoelectronmicroscopic localization of proteasome
(A) Step 19 spermatids. Nuclear staining becomes weaker.Some gold particles are present in clear spots (arrows). Bar = 0.5 µm.
(B) Nuclei of human ejaculated sperm Gold labeling is observed in the clear spots (short arrows) and in the vacuoles (long arrows) but not in the dense matrix. Bar = 0.5 µm.

20S proteasome antibody
Figure 4. Immunoelectronmicroscopic localization of proteasome α2 subunit

Product List

Product Name Cat# Quantity Price

Anti 20S proteasome (GC3(ALPHA))

CAC-SZU-PS-M01 100UL

¥ 50,000
$ 667
€ 500

Anti 20S proteasome (GC3(BETA))

CAC-SZU-PS-M02 100UL

¥ 50,000
$ 667
€ 500

Anti 20S proteasome (GC4/5)

CAC-SZU-PS-M03 100UL

¥ 50,000
$ 667
€ 500

Citation
  • Cytoplasmic lipid droplets are sites of convergence of proteasomal and autophagic degradation of apolipoprotein B.
    Ohsaki Y, Cheng J, Fujita A, Tokumoto T, Fujimoto T.
    Mol Biol Cell 17 2674-2683 (2006) PMID: 16597703


References
  • Identification of the Xenopus 20S proteasome alpha4 subunit which is modified in the meiotic cell cycle. Tokumoto M. et. al., Gene 239 301-308 (1999) PMID: 10548731
  • Two proteins, a goldfish 20S proteasome subunit and the protein interacting with 26S proteasome, change in the meiotic cell cycle. Tokumoto M. et. al., Eur J Biochem 267 97-103 (2000) PMID: 10601855
  • Identification of alpha-type subunits of the Xenopus 20S proteasome and analysis of their changes during the meiotic cell cycle. Wakata Y. et. al., BMC Biochem 5 18 (2004) PMID: 15603592
  • Possible function of caudal nuclear pocket: degradation of nucleoproteins by ubiquitin-proteasome system in rat spermatids and human sperm. Haraguchi CM. et. al., J Histochem Cytochem 55 585-595 (2007) PMID: 17312012

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.